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The results indicate that Nrf2 positively regulates SLC7A11, TXNRD1, GSS, GCL, GSR, GPX4, FSP1, PPP enzymes, and HO-1, enhancing antioxidant capacity and inhibiting ferroptosis (Figure 1D)

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ML226 treatment resulted in HIF-1α stabilization, upregulation of HIF-1 target genes ( CAIX and VEGF ), and activation of a HIF-1 fluorescent reporter (HRE-GFP ODD ) in HeLa cells, similar to treatment with cell-permeable (DM-2OG