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Methods Cell culture Human embryonic kidney (HEK) 293E (ATCC, CRL-1573), Raji cells (ATCC, CCL-86), WSU-NHL (DSMZ GmbH, ACC 58), DOHH-2 (DSMZ GmbH, ACC 47) and SU-DHL-4 (DSMZ GmbH, ACC 495) were maintained in RPMI 1640 medium with L-glutamine (ThermoFisher) all supplemented with 10% heat-inactivated (HI) fetal bovine serum (FCS) (Merck), 25 μg/mL streptomycin and 25 U/mL penicillin (ThermoFisher)
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Microorganisms such as Crysiogenes arsenates, Geospirillum barnesi, Bacillus arsenicoselenatis , and Desulfutomaculum auripigmetum act as arsenate-reducing agents, use As(V) in their respiratory processes [44,132] and release As(III) which being the more soluble form of inorganic As causes As mobilization in soil [44,133]
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