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Txnrd1 siRNAs: GCAUCAAGUUUAUAAGACA, GCGAUAUAUUGGAGGAUAA, CUAAGGAGGCAGCCCAAUA, GGACAGCACAAUUGGAAUC) were obtained from Dharmacon, mouse Slc7a11 siRNA was obtained from Qiagen using the following two sequences: CAACGUUGAUGAUGGACUAT and GAUUUAUCUUCGAUACAAATT Antibodies Anti-Gpx4 (Abcam, ab41787, 1:2000), Anti-xCT (Abcam, ab37185, 1:2000), Anti-Cas9 (Cell signaling, 14697, 1:1000,), Anti-ß-Actin (Sigma, A1978, 1:10,000), Anti-GCLC (Santa Cruz, sc-166345, 1:1000), Anti-Ascl1 (BD Pharmingen, 556604, 1:1000), Anti-Txnrd1 (Cell signaling, 15140S, 1:1000), Anti-Txnrd2 (Cell signaling, 12029, 1:1000), Anti-Acsl4 (Santa Cruz Biotechnology, sc-271800, 1:2000), Anti-Txn1 (Cell signaling, 2429S, 1:1000), Anti-GAPDH (Cell signaling, 97166S, 1:2000), Anti-FSP1 (previously described 55 , kindly provided by M