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In humans, two enzymatic reactions are dependent on vitamin B 12 —methylmalonyl coenzyme A mutase (MUT) reaction and 5-methyltetrahydrofolate-homocysteine methyltransferase (MTR) reaction (Fig 2)

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In order to further solidify their findings, the researchers analyzed the expression of the cell proliferation marker and the proliferating cell nuclear antigen (PCNA) in tendon fibroblasts at the RNA expression level through the use of RT-PCR