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Samples were homogenized with a Qiagen TissueLyser LT for 2 min at 50 Hz, followed by centrifugation at 0 °C for 15 min at 18,000× g (Beckman Coulter, Brea, CA, USA) to pellet proteins and particulates

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Bottle Size:5mL

Key outcome measures included the latency to find the platform during training and the time spent in the target quadrant during probe trials, which are indicators of spatial memory retention [2]