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Inflammasome stimulation To activate the NLRP3 inflammasome, cells were primed for 3 h with LPS (100 ng/mL) followed by addition of 2 µM (BMDMs, peritoneal macrophages, PBMCs), 6µM (THP-1 differentiated macrophages) nigericin (Sigma-Aldrich, N7143-5MG), 2 mM (peritoneal macrophages) ATP or 50 µM (THP-1 differentiated macrophages) R837 (InvivoGen, tlrl-imqs) for 30-60 min

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Classically, IgG-containing immune complexes, such as those formed by IgG binding to spike protein on the surface of infected cells, crosslink FcγRIII on natural killer (NK) cells to elicit ADCC, but neutrophils and macrophages are also capable of comparable cytotoxic functions 28