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Modulation of Enzyme Activity in Phases I and II of the Detoxification Pathway Phases I and II of the detoxification pathway contain the most important enzyme group involved in xenobiotic biotransformation ( Stable ITCs (such as SFN, AITC, PEITC, and BITC), have been found to inhibit carcinogen activation through the inhibition of CYP enzymes in animal studies and cell lines ( in vitro model of cells expressing specific human CYP isoforms, proved that PEITC yielded chemopreventive effects against nitrosamine-induced carcinogenesis through CYP450 inhibition ( Numerous stable ITCs and indole derivatives of GLSs are potent inducers of phase II enzymes ( GST gene that affect their activity have been described ( GSTM1-null and GSTT1-null than among those with normal GST activity ( GSTM1 and GSTT1- null genotype with a significant higher excretion than in those with a normal genotype ( GSTM2, UGT1A1 , and NQO1(NAD(P)H:quinone oxidoreductase 1 , as well as a synergic effect with SFN and PEITC (Figure 3)