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[DOI] [Google Scholar] 133.Ziemann C., Bürkle A., Kahl G.F., Hirsch-Ernst K.I

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The HTRF ratio is then calculated (665 nM/620 nM) for each well to determine the amount of pSTATl in the cell lysate, and the data is then normalized to lOuM (S)-5-((l-(3-oxo-3-(4-(5-(trifhioromethyl)pyrimidin-2- yl)piperazin-l-yl)propoxy)propan-2-yl)amino)-4-(trifluoromethyl)pyridazin-3(2H)-one positive and DMSO negative controls