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Figure 6 When further examining individual variations in trophic positions taking into account COD and VBT within the sub-model (n = 46), the same optimal model emerged, with total body length, season, sex, and the interaction between season and sex as the significant variables, confirming the previous best full-model ( Supplementary Table 11 )

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Then, 200 μL of 0.2M phosphate buffer (pH 7.8) was added to the reaction mixture and incubated at room temperature (25°C) for 15 min