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A more recent work by Torres-Aleman’s group, elegantly demonstrated that this process is initiated by the release of glutamate at active regions, triggering two secondary processes: a vasodilation to increase local serum IGF-I availability and an increased activity of matrix metalloprotease 9, together with cleavage from IGFBP-3

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For the pulmonary fibrosis evaluation, the fibrosis index calculated based on MT staining, collagen deposition and active TGF-β1 expression detected by ELISA, and the expression of TGF-β1, α-smooth muscle actin (SMA), fibronectin, MMP-9, and TIMP-1 by western blotting